Defensive Line (10)
| Pos. | Starter | Backup | Depth |
| DE | Justin Houston | Al-Quadin Muhammad | Tyquan Lewis |
| DT (3-Tech) | DeForest Buckner | Denico Autry | Robert Windsor* |
| DT (1-Tech) | Grover Stewart | Sheldon Day | — |
| DE | Kemoko Turay | Ben Banogu | — |
Analysis: This room will be interesting even if most of the spots are already set. The Colts wisely traded their first-round pick for Buckner and then immediately signed him to a four-year extension. He will command attention at the three-technique. Houston returns in a contract year coming off of an 11.0-sack campaign. The hope is Turay can continue building off of the growth he showed in 2019 before his season was cruelly cut short due to a broken ankle. Right now, he’s the starter opposite Houston. The biggest battle will be between the embattled Lewis and the sixth-round pick in Windsor. If the Colts keep 10 linemen, they are likely safe. It will be interesting to see if the Colts move Lewis to the edge in hopes he can be a strong-side defensive end, but he faces a crucial offseason. Windsor is almost exclusively tied to the interior, but he is also battling for his roster spot.
Notable cuts: Gerri Green